Multiple gene-mediated NAD(P)H-dependent aldehyde reduction is a mechanism of in situ detoxification of furfural and 5-hydroxymethylfurfural by Saccharomyces cerevisiae.
Identifieur interne : 002077 ( Main/Exploration ); précédent : 002076; suivant : 002078Multiple gene-mediated NAD(P)H-dependent aldehyde reduction is a mechanism of in situ detoxification of furfural and 5-hydroxymethylfurfural by Saccharomyces cerevisiae.
Auteurs : Z Lewis Liu [États-Unis] ; Jaewoong Moon ; Brad J. Andersh ; Patricia J. Slininger ; Scott WeberSource :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2008.
Descripteurs français
- KwdFr :
- Aldéhydes (métabolisme), Biotransformation (MeSH), Délétion de séquence (MeSH), Furfural (analogues et dérivés), Furfural (métabolisme), NAD (métabolisme), NADP (métabolisme), Oxydoréduction (MeSH), Protéines de Saccharomyces cerevisiae (génétique), Protéines de Saccharomyces cerevisiae (métabolisme), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (enzymologie), Saccharomyces cerevisiae (métabolisme), Éthanol (métabolisme).
- MESH :
- analogues et dérivés : Furfural.
- croissance et développement : Saccharomyces cerevisiae.
- enzymologie : Saccharomyces cerevisiae.
- génétique : Protéines de Saccharomyces cerevisiae.
- métabolisme : Aldéhydes, Furfural, NAD, NADP, Protéines de Saccharomyces cerevisiae, Saccharomyces cerevisiae, Éthanol.
- Biotransformation, Délétion de séquence, Oxydoréduction.
English descriptors
- KwdEn :
- Aldehydes (metabolism), Biotransformation (MeSH), Ethanol (metabolism), Furaldehyde (analogs & derivatives), Furaldehyde (metabolism), NAD (metabolism), NADP (metabolism), Oxidation-Reduction (MeSH), Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (genetics), Saccharomyces cerevisiae Proteins (metabolism), Sequence Deletion (MeSH).
- MESH :
- chemical , analogs & derivatives : Furaldehyde.
- chemical , genetics : Saccharomyces cerevisiae Proteins.
- chemical , metabolism : Aldehydes, Ethanol, Furaldehyde, NAD, NADP, Saccharomyces cerevisiae Proteins.
- enzymology : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Biotransformation, Oxidation-Reduction, Sequence Deletion.
Abstract
Furfural and 5-hydroxymethylfurfural (HMF) are representative inhibitors generated from biomass pretreatment using dilute acid hydrolysis that interfere with yeast growth and subsequent fermentation. Few yeast strains tolerant to inhibitors are available. In this study, we report a tolerant strain, Saccharomyces cerevisiae NRRL Y-50049, which has enhanced biotransformation ability to convert furfural to furan methanol (FM), HMF to furan di-methanol (FDM), and produce a normal yield of ethanol. Our recent identification of HMF and development of protocol to synthesize the HMF metabolic conversion product FDM allowed studies on fermentation metabolic kinetics in the presence of HMF and furfural. Individual gene-encoding enzymes possessing aldehyde reduction activities demonstrated cofactor preference for NADH or NADPH. However, protein extract from whole yeast cells showed equally strong aldehyde reduction activities coupled with either cofactor. Deletion of a single candidate gene did not affect yeast growth in the presence of the inhibitors. Our results suggest that detoxification of furfural and HMF by the ethanologenic yeast S. cerevisiae strain Y-50049 likely involves multiple gene mediated NAD(P)H-dependent aldehyde reduction. Conversion pathways of furfural and HMF relevant to glycolysis and ethanol production were refined based on our findings in this study.
DOI: 10.1007/s00253-008-1702-0
PubMed: 18810428
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Andersh</name></author><author><name sortKey="Slininger, Patricia J" sort="Slininger, Patricia J" uniqKey="Slininger P" first="Patricia J" last="Slininger">Patricia J. 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Andersh</name></author><author><name sortKey="Slininger, Patricia J" sort="Slininger, Patricia J" uniqKey="Slininger P" first="Patricia J" last="Slininger">Patricia J. Slininger</name></author><author><name sortKey="Weber, Scott" sort="Weber, Scott" uniqKey="Weber S" first="Scott" last="Weber">Scott Weber</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aldehydes (metabolism)</term><term>Biotransformation (MeSH)</term><term>Ethanol (metabolism)</term><term>Furaldehyde (analogs & derivatives)</term><term>Furaldehyde (metabolism)</term><term>NAD (metabolism)</term><term>NADP (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Saccharomyces cerevisiae (enzymology)</term><term>Saccharomyces cerevisiae (growth & development)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Saccharomyces cerevisiae Proteins (genetics)</term><term>Saccharomyces cerevisiae Proteins (metabolism)</term><term>Sequence Deletion (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aldéhydes (métabolisme)</term><term>Biotransformation (MeSH)</term><term>Délétion de séquence (MeSH)</term><term>Furfural (analogues et dérivés)</term><term>Furfural (métabolisme)</term><term>NAD (métabolisme)</term><term>NADP (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Protéines de Saccharomyces cerevisiae (génétique)</term><term>Protéines de Saccharomyces cerevisiae (métabolisme)</term><term>Saccharomyces cerevisiae (croissance et développement)</term><term>Saccharomyces cerevisiae (enzymologie)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Furaldehyde</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aldehydes</term><term>Ethanol</term><term>Furaldehyde</term><term>NAD</term><term>NADP</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Furfural</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aldéhydes</term><term>Furfural</term><term>NAD</term><term>NADP</term><term>Protéines de Saccharomyces cerevisiae</term><term>Saccharomyces cerevisiae</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biotransformation</term><term>Oxidation-Reduction</term><term>Sequence Deletion</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biotransformation</term><term>Délétion de séquence</term><term>Oxydoréduction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Furfural and 5-hydroxymethylfurfural (HMF) are representative inhibitors generated from biomass pretreatment using dilute acid hydrolysis that interfere with yeast growth and subsequent fermentation. Few yeast strains tolerant to inhibitors are available. In this study, we report a tolerant strain, Saccharomyces cerevisiae NRRL Y-50049, which has enhanced biotransformation ability to convert furfural to furan methanol (FM), HMF to furan di-methanol (FDM), and produce a normal yield of ethanol. Our recent identification of HMF and development of protocol to synthesize the HMF metabolic conversion product FDM allowed studies on fermentation metabolic kinetics in the presence of HMF and furfural. Individual gene-encoding enzymes possessing aldehyde reduction activities demonstrated cofactor preference for NADH or NADPH. However, protein extract from whole yeast cells showed equally strong aldehyde reduction activities coupled with either cofactor. Deletion of a single candidate gene did not affect yeast growth in the presence of the inhibitors. Our results suggest that detoxification of furfural and HMF by the ethanologenic yeast S. cerevisiae strain Y-50049 likely involves multiple gene mediated NAD(P)H-dependent aldehyde reduction. Conversion pathways of furfural and HMF relevant to glycolysis and ethanol production were refined based on our findings in this study.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18810428</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>81</Volume><Issue>4</Issue><PubDate><Year>2008</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Multiple gene-mediated NAD(P)H-dependent aldehyde reduction is a mechanism of in situ detoxification of furfural and 5-hydroxymethylfurfural by Saccharomyces cerevisiae.</ArticleTitle><Pagination><MedlinePgn>743-53</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-008-1702-0</ELocationID><Abstract><AbstractText>Furfural and 5-hydroxymethylfurfural (HMF) are representative inhibitors generated from biomass pretreatment using dilute acid hydrolysis that interfere with yeast growth and subsequent fermentation. Few yeast strains tolerant to inhibitors are available. In this study, we report a tolerant strain, Saccharomyces cerevisiae NRRL Y-50049, which has enhanced biotransformation ability to convert furfural to furan methanol (FM), HMF to furan di-methanol (FDM), and produce a normal yield of ethanol. Our recent identification of HMF and development of protocol to synthesize the HMF metabolic conversion product FDM allowed studies on fermentation metabolic kinetics in the presence of HMF and furfural. Individual gene-encoding enzymes possessing aldehyde reduction activities demonstrated cofactor preference for NADH or NADPH. However, protein extract from whole yeast cells showed equally strong aldehyde reduction activities coupled with either cofactor. Deletion of a single candidate gene did not affect yeast growth in the presence of the inhibitors. Our results suggest that detoxification of furfural and HMF by the ethanologenic yeast S. cerevisiae strain Y-50049 likely involves multiple gene mediated NAD(P)H-dependent aldehyde reduction. Conversion pathways of furfural and HMF relevant to glycolysis and ethanol production were refined based on our findings in this study.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Z Lewis</ForeName><Initials>ZL</Initials><AffiliationInfo><Affiliation>US Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL 61604, USA. zlewis.liu@ars.usda.gov</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moon</LastName><ForeName>Jaewoong</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Andersh</LastName><ForeName>Brad J</ForeName><Initials>BJ</Initials></Author><Author ValidYN="Y"><LastName>Slininger</LastName><ForeName>Patricia J</ForeName><Initials>PJ</Initials></Author><Author ValidYN="Y"><LastName>Weber</LastName><ForeName>Scott</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>09</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000447">Aldehydes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0U46U6E8UK</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005662" MajorTopicYN="N">Furaldehyde</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009243" MajorTopicYN="N">NAD</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009249" MajorTopicYN="N">NADP</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>09</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>08</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>9</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>9</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18810428</ArticleId><ArticleId IdType="doi">10.1007/s00253-008-1702-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Illinois</li></region></list><tree><noCountry><name sortKey="Andersh, Brad J" sort="Andersh, Brad J" uniqKey="Andersh B" first="Brad J" last="Andersh">Brad J. Andersh</name><name sortKey="Moon, Jaewoong" sort="Moon, Jaewoong" uniqKey="Moon J" first="Jaewoong" last="Moon">Jaewoong Moon</name><name sortKey="Slininger, Patricia J" sort="Slininger, Patricia J" uniqKey="Slininger P" first="Patricia J" last="Slininger">Patricia J. Slininger</name><name sortKey="Weber, Scott" sort="Weber, Scott" uniqKey="Weber S" first="Scott" last="Weber">Scott Weber</name></noCountry><country name="États-Unis"><region name="Illinois"><name sortKey="Liu, Z Lewis" sort="Liu, Z Lewis" uniqKey="Liu Z" first="Z Lewis" last="Liu">Z Lewis Liu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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